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KMID : 0644120150280020015
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Journal of Oriental Gynecology
2015 Volume.28 No. 2 p.15 ~ p.25
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Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells
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Jang Sae-Byul
Yoo Dong-Youl
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Abstract
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Objectives: In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231.
Methods: The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining.
Results: Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells.
Conclusions: CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.
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KEYWORD
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Chelidonium majus, breast cancer, MDA-MB-231, apoptosis, anti-proliferative
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